Novel oxazine fluorescent dyes for intraoperative neuroimaging / 中国药科大学学报

@#Nerve-specific fluorescent agents can be used as nerve markers in animals to guide surgical procedures and reduce the incidence of intraoperative nerve injury.In this study, the structure of oxazine mother nucleus was modified.A series of oxazine derivative fluorescent dyes YQN-3-YQN-6 were obtained by mass spectrometry and 1H NMR, which can highlight the peripheral nerve structure of rats.Among a series of targeted fluorescent dyes, YQN-3 had emission peaks near NIR and showed highly specific nerve targeting signals in the brachial plexus and sciatic nerves 4 h after intravenous administration.In addition, YQN-3 can accurately locate and identify recurrent laryngeal nerves during thyroidectomy, thus preserving the integrity of these nerves during surgery.With its simple synthesis and low toxicity, YQW-3 can be potentially applied for clinical neural tissue imaging.

Synthesis of high biocompatible nitrogen-doped carbon dots for staining in bio-imaging / 中国药科大学学报

With citric acid as carbon source and different amino acids as nitrogen source,the nitrogen-doped carbon was synthesized by one-step hydrothermal method under the condition of no catalyst.The pre-experiments show that arginine-doped carbon dots(CDs-Arg) with relatively high fluorescence quantum yield (33.25％) were picked out for further study.Furthermore,we studied the physical and chemical properties of CDs-Arg through a series of spectral,electric potential,particle size,X ray and elemental analysis experiments.At the same time,the stabilities of the nanoparticles towards different pH,temperatures,excitation lasers or redox conditions were studied.And the MTr and in vivo distribution experiments were also conducted for the toxicity and metabolism studies.The experimental results showed that the arginine doped carbon dots has high fluorescence efficiency,good stability,and extremely low toxicity.And the water-soluble small particles of CDs-Arg nanoparticles showed that the nanoparticles can be excreted through the glomerulus.These results show that the CDs-Arg Nanoparticles are biocompatible nanoparticles and have potential applications in biological imaging and can serve as a monitor of drug metabolism.

Fabrication, characterization and drug release characteristics of drug loaded poly (L-lactic acid) fiber / 生物医学工程学杂志

To develop a long-term released and implantable biodegradable fiber carrier for hydrophobic drug incorporation, fibers were fabricated by organic phase separation method. The structure of the fiber was observed by scanning electron microscopy and the state of the drug dispersed in the polymer was measured by differential scanning colorimetry (DSC) and Fouvier transform infrared spectroscopy (FTIR). The drug loading content and release profiles were determined by high performance liquid chromatography (HPLC) and ultra-violet spectrophotometry, respectively. Blank and drug loaded fibers were successfully fabricated and the drug was entirely encapsulated into the fiber. The drug was dispersed in the polymer with minicrystal and noncrystal form. The drug release profile was long-term sustained and could be regulated. The PLLA fibers in micrometer range successfully fabricated by organic phase separation method could be taken as sustained release and implantable drug carrier.

Method of localization of microelectrode in the globus pallidus during pallidotomy for Parkinson's disease / 生物医学工程学杂志

Intraoperative analysis of the position of microelectrode in the globus pallidus only depends on experiences and subjective interpretation of microeletrode recording discharge of neurons during pallidotomy for Parkinson's disease. In this paper is reported a method for objective localization of microeletrode during neurosurgery. This method uses the factor of interspike interval based on microelectrode recordings. It is effective for identifying the change of the globus pallidal organism along the microelectrode pathway and the departure or deviation of the needle-pathway. The classification is consistent with the anatomic structures and the results of neurosurgery. This method can be used as a guide in pallidotomy. Globus pallidus.

Value of cardiopulmonary risk index in predicting postoperative short-term prognosis in patients with lung cancer / 中国肺癌杂志

<p><b>BACKGROUND</b>To determine the value of preoperative cardiopulmonary risk index (CPRI) in predicting the short-term prognosis after lung resection in patients with lung cancer.</p><p><b>METHODS</b>Preoperative clinical data were used to generate a cardiac risk index (CRI) and a pulmonary risk index (PRI). And the value of cardiopulmonary risk index (CPRI) consisting of CRI and PRI in predicting postoperative prognosis was estimated in patients who underwent lung resection at Shanghai Chest Hospital in 1999.</p><p><b>RESULTS</b>A total of 625 consecutive patients were studied. Postoperative complications occurred in 49 patients (7.8%), including 8 deaths within 30 days of operation. In the total group, CRI, PRI and CPRI scores ranged from 1 to 3, 0 to 5 and 1 to 7, respectively. There were 489 patients with CPRI < 4, and 136 with CPRI≥4. Using CPRI≥4 as a threshold for predicting postoperative complications, the sensitivity, specificity and accuracy rate were 75.5%, 82.8% and 82.2% respectively.</p><p><b>CONCLUSIONS</b>The preoperative CPRI is one of the important indexes in predicting the short-term postoperative prognosis for patients with lung cancer. However, it can not completely predict all of postoperative risks, and should be used together with other factors.</p>